Journal: bioRxiv

Article Title: Organophosphorus pesticide and nerve agent surrogate metabolism by human CYP3A4

doi: 10.64898/2026.04.23.720309

Figure Lengend Snippet: A. Relative inhibition of CYP3A4 activity using pFluor50 fluorogenic method with DBF as a substrate by thions and oxons at 1 µM concentration. B. Relative inhibition of CYP3A4 activity by thions and oxons at 10 µM concentration. C. Heatmap showing variations in CYP3A4 activity due to OP inhibition at 1 and 10 µM between 10 and 30 min. * Represents OP compounds wherein the inhibition of CYP3A4 changes significantly between 10 and 30 min. CYP3cide was used as positive control for inhibition of CYP3A4.

Article Snippet: The fluorogenic substrate benzyl 2-(6-(benzyloxy)-3-oxo-3H-xanthen-9-yl) benzoate or dibenzyl fluorescein (DBF) for CYP3A4 (# D0282) was purchased from Chemodex (St. Gallen, Switzerland).

Techniques: Inhibition, Activity Assay, Concentration Assay, Positive Control

Journal: bioRxiv

Article Title: Organophosphorus pesticide and nerve agent surrogate metabolism by human CYP3A4

doi: 10.64898/2026.04.23.720309

Figure Lengend Snippet:

Article Snippet: The fluorogenic substrate benzyl 2-(6-(benzyloxy)-3-oxo-3H-xanthen-9-yl) benzoate or dibenzyl fluorescein (DBF) for CYP3A4 (# D0282) was purchased from Chemodex (St. Gallen, Switzerland).

Techniques: Pesticides, Recombinant

Journal: bioRxiv

Article Title: Organophosphorus pesticide and nerve agent surrogate metabolism by human CYP3A4

doi: 10.64898/2026.04.23.720309

Figure Lengend Snippet: A . LC-MS/MS metabolism of oxons by recombinant CYP3A4 after 2 hrs. B . LC-MS/MS degradation of thions by recombinant CYP3A4 after 2 hrs. C . LC-MS/MS metabolism of CWNA surrogates by recombinant CYP3A4 after 1 hr. D . CYP3A4 mediated metabolism of thions was significantly higher as compared to oxons based on mean averages after 1 hr. **P<0.01. Purple circles represent oxons, green circles thions,

Article Snippet: The fluorogenic substrate benzyl 2-(6-(benzyloxy)-3-oxo-3H-xanthen-9-yl) benzoate or dibenzyl fluorescein (DBF) for CYP3A4 (# D0282) was purchased from Chemodex (St. Gallen, Switzerland).

Techniques: Liquid Chromatography with Mass Spectroscopy, Recombinant

Journal: Bioactive Materials

Article Title: Three-dimensional bioprinted hiHeps hepatorganoids with enhanced hepatic functions for the treatment of liver failure and promotion of liver regeneration

doi: 10.1016/j.bioactmat.2025.12.024

Figure Lengend Snippet: Zonation characterization of 3D bioprinted human hepatocyte organoids. A. Representative live/dead assay of hiHep cells in 2D and 3D culture. Scale bar: 200 μm. B. Quantification of cell viability of hiHep cells in 2D and 3D culture (n = 3 per group). C. Cell viability of 2D cultured hiHeps and 3DP-HHO assessed by CCK-8 assay (n = 3 per group). D. Morphological comparison of 2D cultured hiHeps, 3D bioprinted hiHeps organoid, and 3DP-HHO after 1 week/month of culture (Red arrow: liver cord-like structures formed via self-assembly, white arrow: hepatocyte spheroids spontaneously formed by hiHeps). Scale bars: 50 μm. E. Volcano plot of differentially expressed genes in 3DP-HHO compared to 2D cultured hiHeps; key hepatic genes are highlighted. F. KEGG enrichment analysis of up-regulated genes showing biological processes associated with hepatic function. G. Heatmap of zonation marker gene expression (pericentral vs. periportal hepatocyte landmarks) across HepRG, hiHeps, 3DP-HHO, and primary human hepatocytes (PHH). H. Schematic diagram of zonation map illustrating the spatial gene expression of pericentral (red) and periportal (green) markers in healthy liver. I. Representative immunofluorescence images and quantification of CYP3A4 (red), HAL (green), and DAPI (blue) in healthy mouse liver, 2D-cultured hiHeps and 3DP-HHO, demonstrating spatially organized metabolic domains. (White arrow: CYP3A4-positive zones, indicating pericentral hepatocytes, white pentagram: HAL-positive zones, indicating periportal hepatocytes). Scale bars: mouse liver, 100 μm; 3DP-HHO, 25 μm.

Article Snippet: CYP3A4 Mouse mAb, 1:500, 67110-1-Ig, Proteintech, China.

Techniques: Live Dead Assay, Cell Culture, CCK-8 Assay, Comparison, Marker, Gene Expression, Immunofluorescence